Effect of hypoxia on 67Ga incorporation into human granulocytes.
نویسندگان
چکیده
Human granulocytes accumulate 67Ga when incubated under anoxic conditions and exclude the isotope when oxygenated. When incorporated, 67Ga is associated with the lysosomal fraction of the granulocytes. The ability of granulocytes to exclude the isotope except under hypoxic conditions may explain 67Ga localization in abscesses and some tumor masses.
منابع مشابه
Preliminary Dosimetry Study of 67Ga-AATS for Human Based on Biodistribution Data in Rats
Introduction Gallium-67 (67Ga) has been used as a radionuclide for imaging a variety of solid tumors since 1969. Since then use of various gallium-based radiotracers has been reported. Recently, 67Ga-labeled acetylacetate bis(thiosemicarbazones) (67Ga-AATS) complex with significant tumor accumulation and fast blood clearance has been employed. Materials and Methods In this study, the absorbed d...
متن کاملInhibition of human neutrophil chemotaxis by corticosteroids.
A direct inhibitory effect of hydrocortisone on granulocyte chemotaxis has been demonstrated with an increasing inhibition between concentrations of 5 microgram to 12.5 microgram per ml. Washing the granulocytes after incubation with hydrocortisone did not reverse the inhibitory effect on chemotaxis indicating a direct cellular effect. Bacteriocidal capacity of the hydrocortisone treated cells ...
متن کاملEffect of gallium nitrate on the in vivo distribution of 67Ga in rats bearing inflammatory lesions.
Effect of gallium nitrate on the pharmacokinetics and tissue accumulation of 67Ga was investigated in rats bearing turpentine oil-induced abscess. Gallium nitrate accelerated the blood disappearance of 67Ga, but this effect was less potent than that of ferric nitrate. The accumulation of 67Ga in liver was significantly lowered by gallium nitrate, but no significant decrease of 67Ga accumulation...
متن کاملUptake of gallium-67 by the hepatocytes during liver regeneration in carbon tetrachloride-treated mice.
Gallium-67 (67Ga) has been used as a tumor or inflammation-imaging agent in nuclear medicine. We recently reported that the peak of serum alanine aminotransferase (ALT) level was 1 d after carbon tetrachloride (CCl4) treatment in rats. However, the peak of 67Ga uptake by the liver tissue and hepatocytes was 2 d after the treatment. If 67Ga is taken in the hepatic disorder phase, the pattern of ...
متن کاملUptake of gallium-67 by human leukemic cells: demonstration of transferrin receptor-dependent and transferrin-independent mechanisms.
We have studied the role of transferrin and the transferrin receptor in the uptake of 67Ga by the human leukemic cell line HL60. In the absence of transferrin, HL60 cells incorporated about 1% of the 67Ga dose over 6 h. The presence of transferrin increased cellular 67Ga uptake approximately 10-fold. Transferrin-mediated uptake of 67Ga was blocked by an anti-transferrin receptor monoclonal anti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cancer research
دوره 39 12 شماره
صفحات -
تاریخ انتشار 1979